Skip to main content
Fig. 5 | Arthritis Research & Therapy

Fig. 5

From: CircPTPN22 modulates T-cell activation by sponging miR-4689 to regulate S1PR1 expression in patients with systemic lupus erythematosus

Fig. 5

CircPTPN22-miR-4689 regulated T-cell activation via the S1PR1/STAT3 signaling pathway. A, B The expression of S1PR1 mRNA in Jurkat T cells from each group was detected using real-time PCR. C The protein expression of S1PR1 in Jurkat T cells from each group was detected using western blotting. D, E The levels of TNF-α and IL-6 in the supernatant of Jurkat T cells from each group were measured using an ELISA kit. F CCK-8 assay to determine the proliferation of Jurkat T cells transfected with LV-circPTPN22 and miR-4689 mimics or NC mimics. G Flow cytometry analysis of apoptosis in Jurkat T cells transfected with LV- circPTPN22 and miR-4689 mimics or NC mimics. H Flow cytometry analysis of the proportion of cell cycle distribution in Jurkat T cells transfected with LV-circPTPN22 and miR-4689 mimics or NC mimics. The experiments were repeated at least three times. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001

Back to article page

Arthritis Research & Therapy

ISSN: 1478-6362

Contact us