Fig. 3

Immunofluorescence study on the effect of miR-653-5p on chondrocytes. a The protein expression of Col II was detected by the immunofluorescence in miR-653-5p mimics or inhibitor transfected cultured primary human chondrocytes. n = 3 replicates per group. Scale bar = 50 μm. b The protein expression of aggrecan were detected by the immunofluorescence in miR-653-5p mimics or inhibitor transfected cultured primary human chondrocytes. n = 3 replicates per group. Scale bar = 50 μm. c The protein expression of MMP13 was detected by the immunofluorescence in miR-653-5p mimics or inhibitor transfected cultured primary human chondrocytes. n  = 3 replicates per group. Scale bar = 50 μm. d The protein expression of ADAMTS5 was detected by the immunofluorescence in miR-653-5p mimics or inhibitor transfected cultured primary human chondrocytes. n = 3 replicates per group. Scale bar = 50 μm. P values are from one-way ANOVA test followed by Bonferroni’s post hoc test (a, b, c, d). Col II, collagen type II; MMP13, matrix metallopeptidase 13; ADAMTS5, a disintegrin and metalloproteinase with thrombospondin motifs 5