Fig. 6

Effect of miR-653-5p on chondrocyte senescence in DMM animal models. a An overview of the experimental set-up with injections of agomiR-653-5p, antagomiR-653-5p or their negative controls at 1, 2, and 3 weeks after DMM surgery. b In vivo time-dependent fluorescence images in mice at 24, 48, and 72 h after the administration of Cy3-miR-653-5p. c, d Immunofluorescence for p21 and p16 INK4a from the chondrocytes in DMM model treated by agomiR-653-5p, antagomiR-653-5p or their negative controls at 8 weeks. Scale bar = 50 μm. n = 3 mice per group. e SA-β-Gal positivity was analyzed from the chondrocytes in DMM model treated by agomiR-653-5p, antagomiR-653-5p or their negative controls at 8 weeks. n = 3 mice per group. P values are from one-way ANOVA test followed by Bonferroni’s post hoc test (c, d, e). miRNA, microRNA; DMM, destabilization of the medial meniscus; NC, normal control