Fig. 7

Effect of miR-653-5p on OA progression in DMM animal models. a At 8 weeks following DMM surgery, the articular cartilage degeneration was evaluated by H&E staining in different groups treated by agomiR-653-5p, antagomiR-653-5p or their negative controls. n = 6 mice per group. Scale bar = 50 μm. b, c Safranin O-fast green stained sections showed that OA phenotype was significantly alleviated in the DMM-induced OA mice model treated by agomiR-653-5p, as evaluated by OARSI. n = 6 mice per group. d Immunostaining for MMP3 and TNF-α in the DMM model treated by agomiR-653-5p, antagomiR-653-5p or their negative controls at 8 weeks. n = 3 mice per group. Scale bar = 50 μm. P values are from one-way ANOVA test followed by Tukey’s post hoc test (c) and by Bonferroni’s post hoc test (d). miRNA, microRNA; DMM, destabilization of the medial meniscus; MMP3, matrix metallopeptidase 3; TNF-α, tumor necrosis factor-α; OA, osteoarthritis; NC, normal control; OARSI, osteoarthritis research society international