Fig. 1

Generation of deletion of a loxP-flanked HDAC3 target gene in Csf1rCre mice. A C57BL/6J mice expressing Cre-recombinase expressed under the control of the Colony stimulating factor 1 receptor (Csf1r) promoter were mated with HDAC3f/f mice to generate macrophage specific HDAC3 knockout (KO) mice. B Genotyping of transgenic and conditional HDAC3 deletion mice. Resulting PCR products on agarose gel. DNA are extracted from the tail of target mouse and labeled as Tg1 and Tg2. C The BMDMs were represented by positive F4/80 and CD11b and the purity of BMDMs was identified with flow cytometry. D Confirmation of HDAC3 KO in macrophage by western blot. WT: Csf1rCre⁻HDAC3f/f. KO: Csf1rCre⁺HDAC3f/f