Fig. 3

HDAC3 deficiency inhibited MSU crystals-induced inflammatory responses in air pouch model. A Number of total cells in air pouch lavage fluids (APLF) from WT and KO mice after MSU injection at indicated time points. B Flow cytometry analysis of APLF from WT mice and KO mice. Neutrophils were represented by Ly6G⁺, while macrophages were represented by F4/80⁺. C, D Number and ratio of neutrophils (C) and macrophages (D) in APLF from WT and KO mice after MSU injection at indicated time points. E Level of IL-1β in APLF supernatant was measured by ELISA. Data were shown as mean ± SD from three independent experiments (n ≥ 9 mice per group). (*P < 0.05, ***P < 0.001, by unpaired two-tailed Student’s t test)