Fig. 6

Specific HDAC3 inhibitor decreased MSU crystals-induced TNF-α and IL-6 production in BMDMs. A The TNF-α producing BMDMs (represented by F4/80⁺TNF-α⁺) were analysed in WT group and WT treated with HDAC3 inhibitor group (RGFP966). B The Levels of IL-6 were measured by ELISA in supernatant of BMDMs culture medium from WT group and WT treated with HDAC3 inhibitor group (RGFP966). C The schematic diagram showed that HDAC3 deficiency in macrophages inhibited TLR2/4, MyD88, NF-κB, STAT3 and pro-inflammatory cytokines production. Data were shown as mean ± SD from three independent experiments (n = 12 mice per group). (**P < 0.01, by unpaired two-tailed Student’s t test)