Fig. 1

CD11b deficiency exacerbated NLRP3 inflammasome activation in murine macrophages following MSU crystal stimulation. a BMDMs were incubated with 500 μg/ml MSU for 6h and the percentage of BMDMs that took up the MSU crystals was analyzed by flow cytometry based on the increase in the side scatter intensity profile. b and c WT or CD11b KO BMDMs primed overnight with 100 ng/ml of Pam3Cys were stimulated for 6 hours with 500 μg/ml of MSU. IL-1β (b) and IL-6 (e) concentrations were determined by ELISA in cell supernatants. c Western-blot analysis of NLRP3 inflammasome components in supernatants (SN) and in cell lysates of 6 hours MSU-stimulated primed BMDM from WT and CD11b KO mice. d LDH release in cell supernatants from point (b). f Primed BMDMs from WT mice or CD11b-deficient were stimulated with 500 μg/ml of MSU for 2 h. Mitochondrial ROS was measured using MitoSOX. Results in a, b, d, e, and f are expressed as mean ± SD. Data shown represent mean ± SD with group size n=3-6