Fig. 4

Basal pSMAD2/3 is reduced in B27 rat T cells but increases more after TGFβ1 stimuation. (A) Left: Representative histograms of pSMAD2/3 expression in mLN T cells from NTG and B27 rats after 1 h exposure to medium alone (PBS) or TGFβ1 at 37 °C. Right: Intracellular pSMAD2/3 was quantified in mLN T cells from B27 and NTG adult rats after exposure to TGFβ1 or medium alone. Scatter dot plots with bars representing the mean of the SI of 8 experiments. Similar symbols indicate paired conditions. Vertical error bars show the SEM. Paired t-test *: p < 0.05. (B, C) Intracellular pSMAD2/3 was quantified in Tn (B, C) or sorted Teff (C) from B27 and NTG adult rats after exposure to medium (PBS) or TGFβ1 in 8 to 9 independent experiments. Similar symbols indicate paired conditions. (B) Bars height shows the SI mean values. Vertical error bars show the SEM. Paired t-test *: p < 0.05. (C) Mean of pSMAD2/3-fold change after exposure to TGFβ expressed as the ratio of pSMAD2/3 SI after TGFβ1 stimulation over the SI without exposure from (B) (left panel) and from sorted Teff (right panel). Paired t-test *: p < 0.05; ***: p < 0.001, (D) Intracellular pSMAD2/3 was quantified after TGFβ1 stimulation or no stimulation by gating on Tn or Teff from premorbid NTG and B27 rats. Scatter dot plots with bar show the pSMAD2/3 SI. Similar symbols indicate paired conditions. Bars height show the SI mean values (n = 7–9 independent experiments). Vertical error bars show the SEM. Paired t-test *: p < 0.05