Table 1 HLA-B27-specific peptides in Drosophila with their corresponding protein and implication in biological process
Peptide | Second amino acid | Binding affinity (nM) NetMHC 4.0 | Rank NetMHC 4.0* | Protein | Biological process |
|---|---|---|---|---|---|
APGSGAPG | P | 42,924 | 95 | Trailer hitch, isoform E | Cytoskeleton |
EEQEAEVDEN | E | 43350.6 | 99 | Tubulin beta-1 chain | |
GRPLVTMSTY | R | 209.4 | 0.8 | Uncharacterized protein, isoform E | |
LEELQQENCR | E | 40662.7 | 85 | Augmin complex subunit dgt3 | |
TEAPLNPKAN** | E | 42344.2 | 95 | Actin-5 C | |
NRLMYSVIGATEY | R | 4131 | 5 | Z band alternatively spliced PDZ-motif protein 66, isoform I | |
VQYDPRNSETY | Q | 22,065 | 22 | ||
VRYQQQQQQQQQY | R | 4863.8 | 5.5 | Z band alternatively spliced PDZ-motif protein 52, isoform R | |
AQGDFNEFIEK | Q | 26901.4 | 31 | Neuropeptide-like 2 | Immunity |
ARNDPQDDAE | R | 27782.9 | 33 | GH02216p | |
ARNDPQDDAEVIK | R | 13252.4 | 12 | ||
GRITTNQDR | R | 2115.8 | 3.5 | Fat-body protein 1, isoform D | |
QRAMNPVMSI | R | 106.7 | 0.5 | ||
SRMEYSFSNGVVTQ | R | 1483.1 | 3 | Sidestep III, isoform O | |
SRLTYGSNA | R | 1596.4 | 3 | Fondue, isoform A | |
SRLTYGSNAASTF | R | 1088.6 | 2.5 | ||
SSRLTYGSNAASTF | S | 3689.4 | 5 | ||
ARAEINFEGPSP | R | 9277.4 | 8.5 | BcDNA.GH11973 | DNA/RNA |
ARAEINFEGPSPA | R | 4606.2 | 5.5 | ||
ARPYQPDGYQY | R | 13923.4 | 12 | RE50839p | |
NGEFGNELPQRQ | G | 39358.9 | 80 | Vig2, isoform B | |
STITSREIQ | T | 34397.8 | 55 | Histone H2B | |
SVNQQSKTQTVSN | V | 34054.2 | 55 | Protein lingerer, isoform L | |
AAKQTGPIVIS | A | 33461.8 | 55 | 40 S ribosomal protein S19a (Ribosomal protein S19a, isoform C) | Translation |
AGDSKANPPKGAA | G | 36829.9 | 70 | ||
EEIKKEVSS | E | 37291.4 | 70 | ||
GQIANGYTPV | Q | 4211.7 | 5 | Elongation factor 1-alpha | |
NPNQQSERP | P | 37283.8 | 70 | Eukaryotic translation initiation factor 4H1, isoform A | |
VRIGQPIMSV | R | 983.7 | 2.5 | 60 S ribosomal protein L10 | |
DGSVGPVGP | G | 41344.1 | 90 | Nicotinic acetylcholine receptor alpha7, isoform A | Ion transport |
DGSVGPVGPAGP | G | 42269.1 | 95 | ||
HTNNVQEPK | T | 14523.8 | 13 | Calsyntenin-1 | |
TVSGVNGP | V | 39174.1 | 80 | V-type proton ATPase subunit B (Vacuolar proton pump subunit B) | |
YSKTKSAAPNFDE | S | 28671.3 | 35 | GEO09626p1 | |
EGVEGIEHQE | G | 43,278 | 99 | Protein GDAP2 homolog | Stress response |
IEQAGKDATEN | E | 42,286 | 95 | Cytochrome b5 | |
TEDASHMEEVD | E | 42945.4 | 95 | Heat shock protein 83 | |
ERNQNGEDVVK | R | 18,746 | 17 | FI19426p1 | Membrane receptor |
QRVGITAEDL | R | 1000.7 | 2.5 | Msr-110, isoform D | |
YRNAGLYNGNT | R | 8464.9 | 8 | GM18993p | |
YRNAGLYNGNTL | R | 743.8 | 1.8 | ||
YRNAGLYNGNTLVD | R | 566.5 | 1.5 | ||
GRSNISFATSP | R | 7799.6 | 7.5 | Nucleoporin NUP53 | Nuclear membrane |
APSGGPAG | P | 44078.1 | 99 | HECT-type E3 ubiquitin transferase | Ubiquitylation |
GRGNIGQTNY | R | 970.3 | 2.5 | 3-oxoacyl-[acyl-carrier-protein] reductase | Fatty acid metabolism |
SRAPGGAGTGGM | R | 7840.5 | 7.5 | Protein transport protein Sect. 61 subunit beta | Autophagy/ Intracellular protein transport |
GPGAGPGQRP | P | 40685.1 | 85 | FI16123p1 | Unknown |
MRVPPYYLLM | R | 554 | 1.5 | Uncharacterized protein, isoform A | |
MRVPPYYLLMQ | R | 3821.6 | 5 | ||
YRIIESNEVPK | R | 874.3 | 2 | Uncharacterized protein |