Fig. 1

Distribution of B-cell subpopulations in new-onset RA patients (n = 27) and RA patients in remission after JAKi treatment (n = 31) compared to healthy controls (n = 23). (a) The gating strategy for identifying B-cell subpopulations based on the expression of IgD and CD27, which included switched memory (CD27 + IgD-), nonswitched memory (CD27 + IgD+), naive (CD27-IgD+), and DN (CD27-IgD-) B cells. (b) Diverse composition of B-cell subpopulations in the peripheral blood of healthy controls and patients with untreated RA and RA remission after JAKi treatment. (c) Comparative analysis of switched, nonswitched, naive and DNB cells was conducted to evaluate the distribution of the B-cell subpopulation in the peripheral blood of healthy controls and untreated patients and patients with remission of RA after JAKi treatment. Data are shown in box-whisker plots with individual dots, where boxes represent the 25th to 75th percentiles and the lines within the boxes represent the medians. P values were determined with Mann-Whitney U test using GraphPad Prism 6 (**P < 0.01, ***P < 0.001)