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Fig. 1 | Arthritis Research & Therapy

Fig. 1

From: Small heterodimer partner-interacting leucine zipper protein suppresses pain and cartilage destruction in an osteoarthritis model by modulating the AMPK/STAT3 signaling pathway

Fig. 1

Upregulation of SMILE inhibits the progression of OA and cartilage damage in MIA-induced OA in mice. (A) Joint pain was analyzed as the difference between the PWL (left) and PWT (right) in control C57BL/6 MIA-induced OA mice (C57BL/6) and SMILE overexpression vector injected transgenic mice (SMILE Tg). The mice were evaluated for 5 weeks. Nociceptive testing was performed using a dynamic plantar esthesiometer, which is an automated version of the von Frey hair assessment tool. The threshold for pain was shown as weight (g) and relative weight load (%) based on Day 0 as 100%. (B) Safranin O staining of the articular cartilage and tibia tissues of C57BL/6 mice and SMILE Tg. Bar graphs show the average OARSI and Mankin scores. (C) Immunohistochemistry was performed to assess the expression of MCP-1, MMP13 and TIMP3 in articular cartilage of C57BL/6 and SMILE Tg mice. The data are presented as the mean ± standard deviation. Scale bars = 100 μm (B and C). *p < 0.05, **p < 0.01, ***p < 0.001, and ****p < 0.0001

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Arthritis Research & Therapy

ISSN: 1478-6362

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