Fig. 2

Knockdown of WIF-1 in the brain using AAV alleviates abnormal behavior in MRL/lpr mice. (A) Schematic representation of the MRL/lpr mouse model, with AAV administered via intracerebroventricular injection at 9–10 weeks. Behavioral tests were conducted at 13, 16, and 20 weeks, with analysis performed at the conclusion of the final behavioral assessment. (B, C) OFT in the MRL/lpr mouse model at 20 weeks. (D, E) EPM test in the MRL/lpr mouse model at 20 weeks. (F, G) Y-maze test in the MRL/lpr mouse model at 20 weeks. (H) Contextual fear conditioning in the MRL/lpr mouse model at 20 weeks. *: P < 0.05; **: P < 0.01; ***: P < 0.001; ****: P < 0.0001