Fig. 4
From: WIF-1 contributes to lupus-induced neuropsychological deficits via the CRYAB/STAT4-SHH axis
Knockdown of WIF-1 mitigates the proliferation and activation of microglial cells in MRL/lpr mice. (A) tSNE representation highlighting identified hippocampus cell clusters. Flow cytometry quantifies the number of microglial cells, n = 6. (B) Flow cytometry assesses the expression of activation protein CD86 in microglial cells, n = 6. (C) Immunofluorescence co-localization of microglial cells (IBA-1) with PSD95 in the hippocampus, bar = 10 μm, n = 4. (D) WB assesses the expression of LAMP1, IBA-1, and hippocampal synaptic markers PSD95 and SYN1 in the hippocampus. *: P < 0.05; **: P < 0.01; ***: P < 0.001; ****: P < 0.0001