Fig. 5

PUR alleviates abnormal activation of BV2 cells and BBB damage. (A) Correlation between the protein expression level of SHH with microglial ratio of hippocampal samples in two types from lupus mice. (B) Immunofluorescence was used to detect the co-location of SHH and microglia, bar = 10 μm, n = 4. (C, D) Flow cytometry detects the expression of CD86 and LAMP1 in BV2 cells. (E) Flow cytometry evaluates the ability of BV2 cells to engulf yeast glucans. (F) BV2 and HT22 cells were co-cultured at a 1:3 ratio. WB examines the levels of PSD95 and SYN1 expressed by HT22 cells after co-culture. (G) Fluorescence intensity of FITC-Dextran was detected in an in vitro BBB permeability assay. (H) WB assesses the expression levels of ZO1 and Occludin in bEnd.3 cells in an in vitro BBB model. *: P < 0.05; **: P < 0.01; ***: P < 0.001; ****: P < 0.0001