Fig. 6
From: WIF-1 contributes to lupus-induced neuropsychological deficits via the CRYAB/STAT4-SHH axis
CRYAB mediates abnormal activation of BV2 cells. (A) WIF-1 downstream target gene screening strategy map created with BioRender.com. (B) Volcano plot from RNA-seq analysis depicts differentially expressed genes in microglia following rWIF-1 stimulation. (C) Heat map shows the top 10 genes in |log2FC|. (D) WB validates CRYAB expression in the hippocampus of both lupus mouse models and BV2 cells. (E) WB confirms the knockdown effect of si-CRYAB transfection in BV2 cells and assesses the expression of SHH. (F, G) Flow cytometry assesses the expression of CD86 and LAMP1 in BV2 cells. (H) Flow cytometry evaluates the engulfing ability of BV2 cells. *: P < 0.05; **: P < 0.01; ***: P < 0.001