Fig. 8
From: WIF-1 contributes to lupus-induced neuropsychological deficits via the CRYAB/STAT4-SHH axis
SDMA alleviates BV2 activation and BBB damage by inhibiting STAT4. (A) CCK8 assay evaluates SDMA impact on BV2 cell proliferation. (B) WB detects the expression changes in the CRYAB/STAT4-SHH axis after SDMA treatment in BV2 cells. (C, D) Flow cytometry detects the expression of CD86 and LAMP1 in BV2 cells. (E) Flow cytometry assesses the ability of BV2 cells to engulf yeast glucan. (F) WB detects PSD95 and SYN1 expression levels in the co-culture model of cells. (G) WB detects the expression of ZO1 and Occludin in an in vitro BBB model. (H) In vitro BBB permeability assay. *: P < 0.05; **: P < 0.01; ***: P < 0.001; ****: P < 0.0001