Fig. 5

Sema5A promotes Th17 differentiation via PlexinA1. A. CD4⁺ T cells from HC and SLE patients were induced by Th0 cocktail (anti-CD3/CD28 antibody) or Th17 cocktail (anti-CD3/CD28 antibody, IL-1β, IL-6, IL-23, TGF-β1, anti-IFN-γ Antibody, anti-IL-4 antibody co-stimulation), Sema5A or PBS solution was added and cultured for 5 days, the levels of IL-17 A in the culture supernatant were detected by ELISA (n = 6 for HC and n = 8 for SLE patients, Cells from the same donor were paired between different groups). Wilcoxon test, *: P < 0.05, **: P < 0.01; Mann-Whitney U test,: P < 0.05. B. CD4⁺ T cells were activated by anti-CD3/CD28 antibody for 48 h in vitro, and then transfected with universal control siRNA (NC) or siRNA-Plexin A1s, protein expression of PlexinA1 was detected by Western blot. C. CD4⁺ T cells transfected with NC or siRNA-Plexin A1 were cultured with Sema5A or PBS solution for 5 days under Th17-inducing conditions, and the IL-17 A level in the culture supernatant was detected by ELISA. Tukey test *: P < 0.05, **: P < 0.01