Fig. 1

Macrophage depletion experimental design schematic. A Male MaFIA mice had DMM surgery at 12 weeks of age. At 7 weeks after DMM, mice were tested for mechanical allodynia and knee hyperalgesia, followed by 5 once daily injections (i.p.) of vehicle solution or AP20187 (10 mg/kg). Pain-related behaviors were tested afterward, one test per day. Then mice were sacrificed for downstream analyses. B Female MaFIA mice had PMX surgery at 12 weeks of age and were tested for mechanical allodynia, knee hyperalgesia, and static weightbearing at 11 weeks after PMX. After 5 daily i.p. injections of Vehicle or AP20187, mice were tested for the same pain-related behaviors and then sacrificed for downstream analyses