Fig. 3

Acute systemic macrophage depletion does not affect joint damage severity. A Representative Safranin-O stained Vehicle (cartilage degeneration score = 11) or AP20187-treated (cartilage degeneration score = 11) male MaFIA mouse knee images at 8 weeks after DMM surgery; 2x magnification. B Medial cartilage degeneration, (C) Osteophyte width (micrometers), (D) Synovitis medial sum score, (E) Synovial medial hyperplasia, (F) Synovial medial cellularity, and (G) Synovial medial fibrosis scored for male MaFIA mice at 8 weeks after DMM surgery from Vehicle (in blue, n = 10) and AP20187 (in red, n = 13) groups. H Representative Safranin-O stained Vehicle (cartilage degeneration score = 21) or AP20187-treated (cartilage degeneration score = 20) female MaFIA mouse knee images at 12 weeks after PMX surgery; 2x magnification. I – N Same as in (B) – (G) but showing scores for female MaFIA mice at 12 weeks after surgery from Vehicle (in yellow, n = 8) or AP20187 (in purple, n = 10) treated mice. Scale bar is 500 μm. Statistical analysis for cartilage degeneration and osteophyte width by two-tailed unpaired t-test, and for synovitis scoring, Mann-Whitney test. Graphs show mean +/- 95% CI. P values stated on graphs; considered significant if p < 0.05