Acute systemic macrophage depletion in osteoarthritic mice alleviates pain-related behaviors and does not affect joint damage

Geraghty, Terese; Ishihara, Shingo; Obeidat, Alia M.; Adamczyk, Natalie S.; Hunter, Rahel S.; Li, Jun; Wang, Lai; Lee, Hoomin; Ko, Frank C.; Malfait, Anne-Marie; Miller, Rachel E.

doi:10.1186/s13075-024-03457-9

Table 1 Regulation of genes related to immune cell function in the dorsal root ganglia (DRG) in mouse models of OA

From: Acute systemic macrophage depletion in osteoarthritic mice alleviates pain-related behaviors and does not affect joint damage

Gene	From Ref [14]: Early DMM microarray (fold change)	From Ref [14]: Persistent DMM microarray (fold change)	From Ref [39]: MIA model qPCR (fold change)	From Ref [40]: MJL vs. unloaded microarray (fold change)	This Study: DMM veh vs. Naïve veh Bulk RNAseq + 8 weeks (log2(fc))	This Study: DMM AP vs. DMM veh Bulk RNAseq + 8 weeks (log2(fc))
Ccl2 (up in OA)	no change	1.06 (p = 0.06)	1.4221	1.39 (p = 0.02)	1.22 (p = 0.01)	1.14 (p = 0.03)
Ccl3 (up in OA)	no change	no change	1.5999	no change	2.79 (p = 0.009)	no change
Ccl5	1.03 (p = 0.09)	no change	−1.426	no change	no change	no change
Ccl7	no change	no change	no change	no change	1.50 (p = 0.01)	1.14 (p = 0.04)
Ccl12	no change	1.24 (p = 0.03)	−1.5605	no change	no change	1.54 (p = 0.002)
Ccr5 (up in OA)	1.17 (p = 0.08)	1.08 (p = 0.03)	not measured	no change	no change	1.83 (p = 0.00006)
Cx3cr1 (up in OA)	no change	1.26 (p = 0.004)	not measured	no change	1.14 (p = 0.03)	no change
Cxcl10	no change	no change	−2.2689	no change	0.92 (p = 0.07)	−0.97 (p = 0.009)
Cd200r1 (up in OA)	no change	1.03 (p = 0.04)	not measured	no change	1.27 (p = 0.02)	2.28 (p = 3e-7)
Csf1r (up in OA)	1.27 (p = 0.002)	1.14 (p = 0.06)	not measured	no change	no change	no change
Il1𝛃 (up in OA)	no change	1.07 (p = 0.09)	1.5562	no change	1.24 (p = 0.08)	−1.67 (p = 0.03)
Il25 (up in OA)	no change	1.05 (p = 0.07)	not measured	no change	1.92 (p = 0.0007)	no change
Il36g	not present	not present	not measured	not present	1.38 (p = 0.02)	2.33 (p = 0.00002)
Spp1	no change	no change	1.9697	−1.2 (p = 0.09)	no change	no change
S100a8	no change	no change	not measured	no change	1.37 (p = 0.047)	2.93 (p = 2.5e-6)
S100a9	no change	no change	not measured	no change	1.31 (p = 0.05)	2.86 (p = 2.3e-6)
Tlr2	no change	no change	not measured	no change	1.00 (p = 0.09)	1.88 (p = 0.0001)

Bold genes = genes that changed in the same direction in at least 2 mouse models of osteoarthritis
Bold fold change values = genes where DMM AP vs DMM veh regulated genes in the opposite direction as DMM veh vs Naïve veh
DMM destabilization of the medial meniscus, MIA monoiodoacetate, MJL mechanical joint loading, veh vehicle, AP AP20187

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