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Fig. 2 | Arthritis Research & Therapy

Fig. 2

From: Proteomic profiling of human plasma and intervertebral disc tissue reveals matrisomal, but not plasma, biomarkers of disc degeneration

Fig. 2

Discovery proteomic analyses reveal key changes in intervertebral disc (IVD) tissue protein composition associated with increased severity of degeneration. (A) Summary of workflow [1, 2]. Surgically excised disc tissues from patients treated for degenerative disc disorder were formalin-fixed and embedded in paraffin blocks [3]. Tissue sections were collected, stained, and blindly graded by an experienced histopathologist [4]. Tissues graded mild (n = 18) or severe (n = 17) were processed for analysis by liquid chromatography with tandem mass spectrometry (LC/MS/MS). (B) Unsupervised principal component analysis of tissue samples from mild and severe degenerate IVDs. (C) Differential enrichment analysis of protein levels between severe and mild degenerate IVD tissues. (D) Top five enriched KEGG pathways in severe degenerate IVDs in comparison to mild. (E) Plot illustrating proportions of significantly differentially expressed proteins classified into non-matrisome, core matrisome and matrisome-associated proteins

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Arthritis Research & Therapy

ISSN: 1478-6362

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