Fig. 1

sGAG loss of cartilage after enzyme treatment were assessed. (A) Schematic illustration of the platform for ex vivo culture of bovine osteochondral explants and procedures of enzyme treatment. (B) sGAG release. On Day 0 within the CAT group, the initial sGAG content did not originate from zero due to the collection of Aggrecanase buffer for sGAG measurement. (C) Normalization of sGAG retained in cartilage to DNA content. (D) Collagen release (µg). ∗P < 0.05. ∗∗P < 0.01. Triangles, dots or square of different color represent the 4 biological repeats. Abbreviations: CT: Collagenase II treated cartilage, CAT: Collagenase II and Aggrecanase treated cartilage