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Fig. 3 | Arthritis Research & Therapy

Fig. 3

From: Establishment of an ex vivo cartilage damage model by combined collagenase treatment and mechanical loading

Fig. 3

External collagenase II and aggrecanase led to release of inflammatory markers, but mRNA expression of ACAN, COL2A1, SOX9, IL6, ADAMTS4, and ADAMTS5 did not change. (A, B) IL6 and IL-1β release in the medium were detected by ELISA (n = 4). (C) Release of Nitric oxide was detected by Griess reaction (n = 4). (D) RT-qPCR analysis of ACAN, COL2A1, SOX9, IL6, ADAMTS4, and ADAMTS5 (n = 5). Relative quantification of target mRNA was performed according to the 2−ΔΔCt method with RPLP0 as the endogenous control. Gene expression values were normalized to the average values of bovine cartilage at day 0. Triangles, dots or square of different color represent individual donors

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Arthritis Research & Therapy

ISSN: 1478-6362

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