Table 1 Antibodies used for immunohistochemistry staining

COL2A1

ThermoFisher, MA5-12789

Mouse

1 mg/mL pepsin in Tris-HCl, pH 2.0 for 10 min at 37 °C

1/200

ACAN

DSHB, 12/21/1-C-6

Mouse

0.25 U/mL Chondroitinase ABC and 2000 U/mL Hyaluronidase in PBST for 30 min at 37 °C

4 µg/mL

Neo-epitopes of aggrecan

ThermoFisher, MA3-16888

Mouse

0.25U/mL Chondroitinase ABC, 0.05 U/mL Endo beta galactosidase, 0.0005 U/mL Keratanase II in PBST for 30 min at 37 °C

1/50