Table 2 Primers and probes used for PCR
Bovine Gene | Forward primer (5′→3′) | Reverse primer (5′→3′) | Probe (5′→3′) |
|---|---|---|---|
ACAN | CCA ACG AAA CCT ATG ACG TGT ACT | GCA CTC GTT GGC TGC CTC | ATG TTG CAT AGA AGA CCT CGC CCT CCA T (5′FAM/3′TAMRA) |
COL2A1 | AAG AAA CAC ATC TGG TTT GGA GAA A | TGG GAG CCA GGT TGT CAT C | CAA CGG TGG CTT CCA CTT CAG CTA TGG (5′FAM/3′TAMRA) |
SOX9 | AAC GCC GAG CTC AGC AAG | ACG AAC GGC CGC TTC TC | TTC AGC AGT CTC CAG AGC TTG CCC A (5′FAM/3′TAMRA) |
IL6 | TTC CAA AAA TGG AGG AAA AGG A | TCC AGA AGA CCA GCA GTG GTT | CTT CCA ATC TGG GTT CAA TCA GGC GAT T (5′FAM/3′TAMRA) |
ADAMTS4 | CCC CAT GTG CAA CGT CAA G | AGT CTC CAC AAA TCT GCT CAG TGA | AGC CCC CGA AGG GCT AAG CGC (5′FAM/3′TAMRA) |
ADAMTS5 | GAT GGT CAC GGT AAC TGT TTG CT | GCC GGG ACA CAC CGA GTA C | AGG CCA GAC CTA CGA TGC CAG CC (5′FAM/3′TAMRA) |
RPLP0 | Bt03218086_m1 | ||