Fig. 2

CXCL7 enhanced osteoclast differentiation and bone resorption in RA patients. CD14 + monocytes isolated from RA patients were cultured with M-CSF, RANKL and CXCL7 at specified concentrations. (A )and (B). Representative images of TRAP and F-actin staining. (C). Exemplary scanning electron microscopy images of bone resorption pits. The proportion of resorption pit area in 5 random fields per group was measured. (D). The ratio of osteoclast number to control group in 5 random fields each well in TRAP staining. (E). The ratio of F-actin ring number to control group in 5 random fields each well in F-actin staining. F. Transcription levels of TRAP, cathepsin K, and RANK after 3 days. Each group consisted of three samples. Data are presented as mean ± SD. Statistical analyses were conducted using one-way ANOVA. *P < 0.05, **P < 0.01, ***P < 0.001. Scale bar in A is 200 μm. Scale bar in B is 100 μm. Scale bar in C is 10 μm