Fig. 3

Cxcl7 enhanced osteoclast differentiation via ERK/NFATc1 signaling pathways in RAW264.7 cells. (A) RAW264.7 cells were cultured in the presence of M-CSF and RANKL with Cxcl7 at the indicated concentrations. After 5 days, cells were fixed and stained for TRAP. Number of osteoclasts and number of nuclei in each osteoclast were counted. (B) After 3 days, transcription levels of TRAP, cathepsin K, and RANK were detected. (C) Cxcl7 increased the activation of the p-ERK at 10 and 15 min, with no effect on p-p65. (D) Cxcl7 induced the mRNA expression of NFATc1 in RAW264.7 cells at 3 days, with no effect on c-Fos. Values shown represent the mean ± SD from three separate experiments. Statistical analyses were conducted using one-way ANOVA. *P < 0.05, **P < 0.01, ***P < 0.001. Scale bar is 100 μm