Fig. 5

Resistin promotes inflammation and catabolism in MetS-KOA-FLS. MetS-KOA-FLS were stimulated with or without 200 ng/ml resistin for 24 h. (Ctrl: 0 ng/ml resistin, Resistin: 200 ng/ml resistin, n = 5.) a, Relative mRNA levels of CCL3, ICAM1, TNFα, MMP13, and ADAMTS4 in MetS-KOA-FLS treated with or without resistin were detected by qRT-PCR. b, MetS-KOA-FLS were treated with resistin for 24 h, and then the protein levels of CCL3, MMP13, and ADAMTS4 in the culture supernatant were measured by ELISA c, MetS-KOA-FLS stimulated with or without 200 ng/ml resistin for 24 h were then co-cultured (1:3) with THP-1 cells for 6 h. The number of adherent THP-1 cells was shown on the right. d, MetS-KOA-FLS stimulated with and without 200 ng/ml resistin for 72 h were subjected to invasion assay. e, mRNA and protein level of CPT1A in MetS-KOA-FLS treated with or without resistin were detected by qRT-PCR and western blot. f, Relative mRNA levels of HK and LDHA in MetS-KOA-FLS treated with or without resistin were detected by qRT-PCR. g, Protein levels of HK2 and LDHA in MetS-KOA-FLS treated with or without resistin were detected by western blot. Data are expressed as means ± s.e.m. Statistical significance indicated as *P < 0.05, and**P < 0.01, and ***P < 0.001, as assessed by matched samples t- test