Fig. 7

Resistin promotes the expression of CPT1A through CAP1/PKA/CREB. a In vitro protein and mRNA levels of TLR4 in nMetS-KOA-FLS and MetS-KOA-FLS. b In vitro protein and mRNA levels of CAP1 in nMetS-KOA-FLS and MetS-KOA-FLS. c Immunohistochemistry of CAP1 in synovium of nMetS-KOA and MetS-KOA. d, mRNA and protein levels of CPT1A in MetS-KOA-FLS pretreated with 10 nM TAK-242 for 1 h and then incubated with 200 ng/ml resistin for 24 h. e mRNA and protein levels of CAP1 in MetS-KOA-FLS 48 h after transfection with CAP1-siRNA. f Protein levels of CAP1 and CPT1A in MetS-KOA-FLS transfected with CAP1-siRNA for 48 h and incubated with 200 ng/ml resistin for 24 h. g mRNA levels of CPT1A in MetS-KOA-FLS transfected with CAP1-siRNA for 48 h and incubated with 200 ng/ml resistin for 24 h. h Protein levels of CREB and pCREB^ser133 in MetS-KOA-FLS transfected with CAP1-siRNA for 48 h and incubated with 200 ng/ml resistin for 24 h. i mRNA level of CPT1A in MetS-KOA-FLS pretreated with 2.5 µM KG-501 or 2.5 µM H-89 for 1 h and then incubated with 200 ng/ml resistin for 24 h. j Protein levels of CREB, pCREB^ser133, and CPT1A in MetS-KOA-FLS pretreated with 2.5 µM KG-501 or 2.5 µM H-89 for 1 h and then incubated with 200 ng/ml resistin for 24 h. k mRNA levels of CCL3, ICAM1 and TNFα in MetS-KOA-FLS pretreated with 2.5 µM KG-501 or 2.5 µM H-89 for 1 h and then incubated with 200 ng/ml resistin for 24 h. l mRNA levels of MMP13 and ADAMTS4 in MetS-KOA-FLS pretreated with 2.5 µM KG-501 or 2.5 µM H-89 for 1 h and then incubated with 200 ng/ml resistin for 24 h. Data are expressed as means ± s.e.m. n = 5 biologically independent samples of MetS-KOA-FLS. Statistical significance indicated as *P < 0.05, **P < 0.01, and ***P < 0.001, as assessed by matched samples t- test