Fig. 8

Validation of maker genes associated with ferroptotic NP cell subsets. A-B The primary NP cells cultured in the three groups were incubated with PBS, DMSO or DMSO + Erastin for 24 h, respectively, and then the cells were collected. The mRNA expression levels of Rps6 (A) and Cxcl1 (B) were detected by Real-Time PCR. Data are expressed as the mean ± SEM (n = 3). (C-D) Immunofluorescence staining (Rps6 and Acsl4) of 10 and 20M-old rat tail IVD cross sections. Scale bars, 500 µm (C), Scale bars, 50 or 10 µm (D). E Immunofluorescence staining (Cxcl1 and Acsl4) of 10 and 20M-old rat tail IVD cross sections. Representative images of enlarged NP cells are shown on the right. Scale bars, 50 or 10 µm. **P < 0.01, NS, no significance