Fig. 1
From: Atf3 + senescent chondrocytes mediate meniscus degeneration in aging
Histological analysis reveals age-related changes in mice meniscus (A) Safranin O/Fast Green staining of articular cartilage in young and aged rats. Scoring was performed independently by two experienced pathologists in a blinded manner to ensure objective evaluation. Scale bar: 100 μm (B) Representative hematoxylin and eosin (H&E) staining of synovial tissue in young and aged rats, showing age-associated synovial inflammation. Scale bar: 100 μm. (C) Quantification of the OARSI score and synovitis score in young and aged rats. (D, E) Micro-CT evaluation of the tibial subchondral bone revealed significant age-related changes. (D) Representative 3D reconstructed images of the tibial subchondral bone from young (YM) and aged (AM) rats. (E) Quantitative analysis of trabecular thickness (Tb.Th) and bone volume fraction (BV/TV) in the young and aged group. Scale bars: 5 mm.Statistical significance was assessed using one-way ANOVA followed by Tukey’s multiple comparison test (*p < 0.05, **p < 0.01, ***p < 0.001)