Fig. 4
From: Atf3 + senescent chondrocytes mediate meniscus degeneration in aging
The Atf3 + subset identified as a senescent chondrocyte population. (A, B) Gene Ontology(A) enrichment and KEGG analysis(B) for differentially expressed genes in Atf3 + chondrocytes of meniscus. X-axis:–log10(p-value);p < 0.001 (C) Heatmap showing the enrichment of key biological pathways in chondrocyte subpopulations. (D, E) Representative immunohistochemistry (IHC) staining of Atf3 in young and aged mouse menisci, and quantification of Atf3-positive cells. Scale bar: 100 μm. Con, n = 6; Aged, n = 6. ****p < 0.0001. (F) The relative expression levels of Atf3 were quantified by qPCR, demonstrating differential expression between young and aged mice. Con, n = 6; Aged, n = 6. *p < 0.05 (G) Boxplot of single-sample Gene Set Enrichment Analysis (ssGSEA) of each chondrocyte subset. ***p < 0.001 (H) CytoTRACE analysis of the three subsets.***p < 0.001 (I) Pseudotime progression diagram, with color from dark to light indicating the pseudo-time order (top). The pseudo-temporal map of chondrocytes is colored according to cell subtype (bottom)