Fig. 5
From: Atf3 + senescent chondrocytes mediate meniscus degeneration in aging
Alterations in individual signaling pathways in aging and young chondrocyte groups. (A) Information flow among young and aged meniscus chondrocytes. Pathways in red are enriched in the aging meniscus chondrocytes, while those in green are enriched in the young meniscus chondrocytes. (B) Bar chart displaying differences in the relative ratio of information flow in the interaction network between the aging and young meniscus chondrocytes. (C) Degree and differences of the ANGPTL signaling pathway network in chondrocytes from the young (top) and aged (bottom) meniscus. (D) The most prominent receptor-ligand pair contributing to the ANGPTL pathway in young and aged groups. (E) Violin plots showing the expression of Angptl4 in aged vs. young groups (top) and among three chondrocyte subsets (bottom). (F, G) Representative immunohistochemistry (IHC) staining of Angptl4 in young and aged mice meniscus, and quantification of Angptl4-positive cells. Scale bar: 100 μm. Con, n = 6; Aged, n = 6. ****p < 0.0001. (H) The relative expression levels of Angptl4 were quantified by qPCR, demonstrating differential expression between young and aged mice. Con, n = 6; Aged, n = 6. ***p < 0.001