Fig. 3

The endocytosis of chondrocyte lysates by FLSs is regulated through CAV1, and CLs downregulate the expression of CAV1 in FLSs. (A). qRT‒PCR analysis of CAV1 in FLSs with and without CL pretreatment (n = 6). (B). WB and quantification of CAV1 levels in FLSs with or without CL pretreatment (n = 3). (C). Representative coimmunofluorescence images of CAV1 (green fluorescence) and CLs (red fluorescence). (D). Representative coimmunostaining images for CAV1 and NLRP3 in FLSs with or without CL pretreatment after transfection with si-CAV1 or a control. (E). WB and quantitative analysis of ERK and p-ERK levels in FLSs with or without CL pretreatment. (F). WB and quantitative analysis of CAV1, ERK and p-ERK levels in CL-stimulated FLSs with or without hypaphorine treatment (n = 3). All the data are presented as the means ± SEMs. Paired t tests and one-way analysis of variance (ANOVA) were used for statistical analysis. * p < 0.05, ** p < 0.01, ***p < 0.001. FLSs, fibroblast-like synoviocytes; NA, normal articular; OA, osteoarthritis