Fig. 5

CAV1 overexpression in FLSs alleviates synovitis in a DMM-induced OA model through the regulation of NLRP3 inflammasome-mediated pyroptosis. (A). Representative images of HE-stained knee joints from C57BL/6J (wild-type) DMM mice and NLRP3 knockout DMM mice with or without intra-articular injection of AAV-CAV1 (oeCAV1) and quantification of the synovitis score (n = 5). (B). Representative images of coimmunofluorescence of CAV1 (red fluorescence) and vimentin (green fluorescence) in knee joints from C57BL/6J (wild-type) DMM mice with or without intra-articular injection of AAV-CAV1 (n = 5). (C). Representative images of NLRP3 (red fluorescence) and vimentin (green fluorescence) in knee joints from C57BL/6J (wild-type) DMM mice with or without intra-articular injection of AAV-CAV1 (n = 5). (D). Representative images of coimmunofluorescence of CAV1 (red fluorescence) and vimentin (green fluorescence) in the knee joints of NLRP3 knockout mice with or without intra-articular injection of AAV-CAV1 (n = 5). All the data are presented as the means ± SEMs. Paired t tests and one-way analysis of variance (ANOVA) were used for statistical analysis. * p < 0.05, ** p < 0.01, ***p < 0.001. HE, haematoxylin‒eosin; DMM, destabilization of the medial meniscus; AAV, adeno-associated virus. KO, knockout